Untargeted Metabolomic Analysis Based on DCL™

Background & Overview

The ultimate goal of metabolomics is the comprehensive study of low molecular weight molecules in living organisms. Metabolites are the result of biological and environmental factors and, therefore, offer great potential for bridging genotypic and phenotypic knowledge. Untargeted metabolomics, also known as discovery metabolomics, is used when there is no intended research target. Its characteristic is to characterize as many metabolites as possible and cover more metabolic pathways. BOC Sciences' non-targeted metabolomics based on deuterium isotope labeling combined with liquid chromatography-mass spectrometry (LC-MS) enables accurate and rapid analysis of various biological samples for disease diagnosis, pathological research, new drug development, drug toxicology, etc. Offers new perspectives for exploration.

Analysis platform

Sample processing

For labile hydrogen atoms in functional groups such as hydroxyl, carboxyl, or amide, the hydrogen atoms are replaced by reacting the compound with deuterium reagents such as D₂O and ND₃ in the liquid or gas phase. The resulting deuterated samples are analyzed using LC-MS in full scan mode, and the number of labile hydrogen atoms on the corresponding functional groups in the molecule can then be counted, yielding structural information about the metabolites detected in complex mixtures. It is also possible to first perform hydrogen-deuterium exchange on the target compound and then analyze deuterated compounds by MS², where the rearrangement of unstable hydrogen atoms and deuterium atoms occurs with the added energy.

Data processing

The data processing workflow includes noise filtering, peak detection, peak deconvolution, retention time alignment, and finally feature annotation.

Technical Advantages

Mass spectrometry platform combined with deuterium isotope labeling

Facilitates metabolite identification in untargeted metabolomics and can potentially be extended to other types of metabolites

Wide range of assays

Widely used in environmental analysis, nutritional research, gut microbe-host interactions, biomarker discovery, identification of active metabolites, etc.

Advanced technology

Collect data without pre-existing knowledge

Strict QC

Adopt the most stringent QC system to ensure qualitative and quantitative results for each item

Professional services

Professional, reliable and guaranteed pre-sales and after-sales, and responsible for data publication to the end

Fig.1 Exclusion of unreasonable candidates using HDS–CIL–LC–MS.^[1]

Application Fields

• Agriculture and forestry field: stress resistance mechanism, growth and development mechanism, breeding and protection research, etc.
• Basic medicine, clinical diagnosis: biomarkers, disease mechanism, disease classification, personalized treatment, etc.
• Biomedicine: mechanism of drug action, drug efficacy evaluation, drug development, etc.
• Microbial field: pathogenic mechanism, drug resistance mechanism, pathogen-host interaction research, etc.
• Bioenergy, environmental science field: optimization of fermentation process, biofuel production, etc.

If you are interested in our services, please contact us immediately, then fill in the complete inquiry form, and we will reply to you as soon as possible.

Reference

1. Zheng S J, et al. Hydrogen–deuterium scrambling based on chemical isotope labeling coupled with LC–MS: Application to amine metabolite identification in untargeted metabolomics. Analytical chemistry. 2020, 92(2): 2043-2051.