Accurate determination of total body water is a routine clinical measurement and an important indicator for body fat evaluation. Examples of compounds most commonly used to measure total body water (TBW) are antipyrene, tritium oxide, and deuterium oxide (D2O). Antipyrene is metabolized and selectively bound to proteins, while tritiated water is a radiotracer. Therefore, heavy water is used as a tracer to measure the total water content of the human body. There are many methods for the determination of deuterium in water in vivo, including drop method, freezing point elevation method, and gas chromatography. But these methods often lack the necessary sensitivity and are not widely used. Fourier transform infrared spectroscopy (FT-IR) has become the method of choice due to its relatively high sensitivity and technical simplicity.
BOC Sciences has developed an improved and inexpensive infrared absorption method based on Fourier transform infrared spectroscopy (FT-IR) for measuring TBW using D2O tracer dosimetry. Our advantage is providing shorter analysis times while requiring smaller sample volumes.
In conventional FTIR spectroscopy methods, deuterium quantification is achieved by measuring the absorption of the O-D stretch peak. The FT-IR technique measures the absorption spectrum in this region, which can more accurately quantify the absorption of the O-D bond by accounting for the interference from the larger signal from the H-O bond. FT-IR is usually performed in transmission mode, where deuterium is quantified by absorption of the infrared signal through a sample contained in cells. A larger sample volume is usually required to ensure that the cell is completely filled with the sample being analyzed.
Instead, we are using Attenuated Total Reflection Fourier Transform Infrared Spectroscopy (ATR-FTIR), by measuring the changes in absorption signal that occur in a totally internally reflected infrared beam, which produces an evanescent wave that extends slightly beyond the surface of an optically dense crystal of high refractive index into a sample in contact with the crystal surface.
Our method is a fast and simple method for measuring deuterium in plasma, which requires only a very small sample volume and analyzes the samples with high accuracy (CV < 0.5 %).
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