Quantitative Proteomics Based on DCL™
Background & Overview
The purpose of quantitative proteomics is to identify all proteins in a complex mixed system, and to accurately measure the amount and amount of proteins, which is an important part of current systems biology research. In recent years, due to the advancement of mass spectrometry and bioinformatics, quantitative proteomics has achieved remarkable achievements in the analysis of proteome or sub-proteome, but its most notable achievements should be attributed to the application of stable isotope labeling technology . This technology uses chemical probes specific for a certain type of protein to label the target protein or peptide, and the chemical probes are required to contain stable isotope signals for accurate quantification. On this basis, precise quantitative analysis of expressed protein differences and post-translationally modified protein differences was achieved. BOC Sciences provides marker quantitative proteomic analysis services based on mass spectrometry and DCL™ technology.
Analysis platform
Our DCL™ technology provides novel deuterated alkylating agents for quantitative proteomics with excellent performance on electrospray ionization-based mass spectrometry instruments, providing a cost-effective technique for large-scale quantitative proteomic measurements.
Fig.1 Label workflow.
Protein Labeling
Proteins were dissolved in buffer and digested overnight. The digest was mixed with the prepared deuterated reagent and incubated at room temperature for a certain period of time before adding a stop reagent to stop the reaction. After acidification, incubation, washing and other steps, the entire sample processing process is completed.
Liquid Chromatography-Mass Spectrometry/Mass Spectrometry
The labeled peptides were separated by reversed-phase chromatography on a nano-HPLC. The mass spectrometer was then run in positive ion mode to complete the MS/MS acquisition with data correlation.
Data Analysis
MS acquisition files can be processed using proteome discovery software, and the processed data are then submitted to a custom server for peptide identification and quantification.
Technical Advantages
- Label multiple samples simultaneously. High-throughput detection of multiple samples with more flexibility in experimental design
- In vitro labeling at the peptide level for many types of biological samples
- Good reproducibility and high sensitivity, can be qualitative and quantitative at the same time
Application Fields
Areas of application for our quantitative proteomic analysis based on DCL™ include, but are not limited to:
- Mechanism and regulatory mechanism research
- Mechanism research in dynamic process
- Screening for disease markers
- Medication and prognostic marker screening
- Drug target research
- Special function protein screening
BOC Sciences can provide one-stop analytical services and complete project reports. Our reports include relevant mass spectrometry parameters, raw data, mass spectrometry images, differential protein level analysis, and bioinformatics analysis.
Our complete instrument platform, strong technical team and data analysis capabilities, and considerate after-sales service guarantee high-quality service quality. If you are interested in our services, please contact us immediately, then fill in the complete inquiry form, and we will reply to you as soon as possible.
Reference
- Zhang J, et al. Deuterium isobaric amine-reactive tags for quantitative proteomics. Analytical chemistry. 2010, 82(18): 7588-7595.
