Glycans are complex structures made up of simple sugars linked together. Human glycomics contains millions of different glycan species. Glycans are involved in a range of key biological processes, such as maintaining tissue elasticity, fighting pathogens, and regulating the immune system. Glycomics can reflect various events that occur at the molecular level in cells. Therefore, taking advantage of this enormous diversity of glycans, highly specific diagnostic methods can be developed to provide very detailed information. In particular, quantifying changes in glycan composition and abundance is critical for elucidating the physiopathology of diseases caused by aberrant glycosylation.
Quantitative analysis of glycans can be performed by incorporating stable isotopes into derivatizing reagents. Therefore, a number of chemical and enzymatic isotope labeling techniques have been developed for quantitative glycomics. BOC Sciences has developed an alternative partially metabolized isotope labeling platform, DCL™, for the relative quantification of glycans. Our technique can incorporate D into any biomolecule-involving metabolic pathway for water, suitable for quantitative multi-omics.
Our mission is to provide useful solutions that make glycomics research easier for professionals and non-professionals alike.
Our services can be used to identify all glycans expressed in plasma/serum, cells, tissues, or organisms. We introduce low concentrations of D2O into biological systems to induce partial D labeling on non-labile C-H bonds in biomolecules through water-related metabolic processes; D can also be bound enzymatically to C-H bonds in ribose or deoxyribose middle. Glycans were collected by solid-phase extraction using optimized procedures and finally quantified.
The general steps of quantitative glycomics analysis are as follows:
Fig.1 Glycomic and glycoproteomic strategies for glycan analysis.
BOC Sciences provides efficient deuterium-labeled mass spectrometry support for glycomics research, our service areas include but are not limited to:
A deuterium labeling-based quantitative technique developed by BOC Sciences can be used for quantitative analysis of glycomics. Our method is used to perform quantitative glycomics with a dynamic range over 2 orders of magnitude with reliable accuracy and precision.
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