Quantitative Glycomics Based on DCL™
Background & Overview
Glycans are complex structures made up of simple sugars linked together. Human glycomics contains millions of different glycan species. Glycans are involved in a range of key biological processes, such as maintaining tissue elasticity, fighting pathogens, and regulating the immune system. Glycomics can reflect various events that occur at the molecular level in cells. Therefore, taking advantage of this enormous diversity of glycans, highly specific diagnostic methods can be developed to provide very detailed information. In particular, quantifying changes in glycan composition and abundance is critical for elucidating the physiopathology of diseases caused by aberrant glycosylation.
Quantitative analysis of glycans can be performed by incorporating stable isotopes into derivatizing reagents. Therefore, a number of chemical and enzymatic isotope labeling techniques have been developed for quantitative glycomics. BOC Sciences has developed an alternative partially metabolized isotope labeling platform, DCL™, for the relative quantification of glycans. Our technique can incorporate D into any biomolecule-involving metabolic pathway for water, suitable for quantitative multi-omics.
Our Services
Our mission is to provide useful solutions that make glycomics research easier for professionals and non-professionals alike.
Our services can be used to identify all glycans expressed in plasma/serum, cells, tissues, or organisms. We introduce low concentrations of D2O into biological systems to induce partial D labeling on non-labile C-H bonds in biomolecules through water-related metabolic processes; D can also be bound enzymatically to C-H bonds in ribose or deoxyribose middle. Glycans were collected by solid-phase extraction using optimized procedures and finally quantified.
The general steps of quantitative glycomics analysis are as follows:
- Cell Culture and Sample Preparation
- Separation of Glycoprotein and/or Glycolipid Fractions
- N-, O- and/or Glycosphingolipid Release and Enrichment
- Identification of Polysaccharides by Liquid Chromatography-MS
- Quantitative Analysis of Glycans
Fig.1 Glycomic and glycoproteomic strategies for glycan analysis.[2]
Application Fields
BOC Sciences provides efficient deuterium-labeled mass spectrometry support for glycomics research, our service areas include but are not limited to:
- Basic and Clinical Research
- agriculture
- Food and Nutrition
- Pharmaceutical Industry
- Biofuels
A deuterium labeling-based quantitative technique developed by BOC Sciences can be used for quantitative analysis of glycomics. Our method is used to perform quantitative glycomics with a dynamic range over 2 orders of magnitude with reliable accuracy and precision.
If you are interested in our services, please contact us immediately, then fill in the complete inquiry form, and we will reply to you as soon as possible.
References
- Kim J, et al. Deuterium Oxide Labeling for Global Omics Relative Quantification (DOLGOReQ): Application to Glycomics. Analytical Chemistry. 2021, 93(43): 14497-14505.
- Mereiter S, et al. Glycomic approaches for the discovery of targets in gastrointestinal cancer. Frontiers in oncology. 2016, 6: 55.
